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streptavidin pulldown  (New England Biolabs)


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    New England Biolabs streptavidin pulldown
    Streptavidin Pulldown, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 4080 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptavidin+pulldown/Streptavidin/pmc12977078-147-2-9
    Average 97 stars, based on 4080 article reviews
    streptavidin pulldown - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism.
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37 ◦C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg / ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37 ◦C for an additional 30 min with mixing. ..

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg/ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37°C for an additional 30 min with mixing. ..

    Article Title: Long G4-rich enhancer physically interacts with EXOC3 promoter via a G4:G4 DNA-based mechanism
    Article Snippet: 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593-031) extraction. .. To perform streptavidin pulldown, 120 μL (4 mg/mL) streptavidin magnetic beads (NEB cat no. 50-812-660) were washed 3 times with 1 mL of lysis buffer then resuspended in 100 μL of complete lysis buffer and added to the hybridization mix and then incubated at 37 °C for an additional 30 min with mixing. ..

    Lysis:

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism.
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37 ◦C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg / ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37 ◦C for an additional 30 min with mixing. ..

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg/ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37°C for an additional 30 min with mixing. ..

    Article Title: Long G4-rich enhancer physically interacts with EXOC3 promoter via a G4:G4 DNA-based mechanism
    Article Snippet: 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593-031) extraction. .. To perform streptavidin pulldown, 120 μL (4 mg/mL) streptavidin magnetic beads (NEB cat no. 50-812-660) were washed 3 times with 1 mL of lysis buffer then resuspended in 100 μL of complete lysis buffer and added to the hybridization mix and then incubated at 37 °C for an additional 30 min with mixing. ..

    Hybridization:

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism.
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37 ◦C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg / ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37 ◦C for an additional 30 min with mixing. ..

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg/ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37°C for an additional 30 min with mixing. ..

    Article Title: Long G4-rich enhancer physically interacts with EXOC3 promoter via a G4:G4 DNA-based mechanism
    Article Snippet: 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593-031) extraction. .. To perform streptavidin pulldown, 120 μL (4 mg/mL) streptavidin magnetic beads (NEB cat no. 50-812-660) were washed 3 times with 1 mL of lysis buffer then resuspended in 100 μL of complete lysis buffer and added to the hybridization mix and then incubated at 37 °C for an additional 30 min with mixing. ..

    Incubation:

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism.
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37 ◦C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg / ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37 ◦C for an additional 30 min with mixing. ..

    Article Title: Long G4-rich enhancers target promoters via a G4 DNA-based mechanism
    Article Snippet: A total of 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593–031) extraction. .. To perform streptavidin pulldown, 120 μl (4 mg/ml) streptavidin magnetic beads (NEB cat no. 50–812-660) were washed three times with 1 ml of lysis buffer then resuspended in 100 μl of complete lysis buffer and added to the hybridization mix and then incubated at 37°C for an additional 30 min with mixing. ..

    Article Title: Phosphorylation of serine-893 in CARD11 suppresses the formation and activity of the CARD11-BCL10-MALT1 complex in T and B cells.
    Article Snippet: CARD11 acts as a gatekeeper for adaptive immune responses after T cell or B cell antigen receptor (TCR/BCR) ligation on lymphocytes.. PKC / -catalyzed phosphorylation of CARD11 promotes the assembly of the CARD11-BCL10-MALT1 (CBM) complex and lymphocyte activation.. Here, we demonstrated that PKC / -dependent CARD11 phosphorylation also suppressed CARD11 functions in T or B cells.

    Article Title: Long G4-rich enhancer physically interacts with EXOC3 promoter via a G4:G4 DNA-based mechanism
    Article Snippet: 100 pM of Biotinylated probes (Integrated DNA Technologies Coralville, IA) antisense to the Chr5LG4 (primer probe table) or non-targeting control probes (or no probes for input DNA control) were added to the hybridization mix and incubated at 37°C for 4 h in an orbital shaker (250 rpm) following this step the sample designated for input DNA was isolated by phenol-chloroform isoamyl alcohol 25:24:1 (Invitrogen cat no. 15593-031) extraction. .. To perform streptavidin pulldown, 120 μL (4 mg/mL) streptavidin magnetic beads (NEB cat no. 50-812-660) were washed 3 times with 1 mL of lysis buffer then resuspended in 100 μL of complete lysis buffer and added to the hybridization mix and then incubated at 37 °C for an additional 30 min with mixing. ..

    Phospho-proteomics:

    Article Title: Phosphorylation of serine-893 in CARD11 suppresses the formation and activity of the CARD11-BCL10-MALT1 complex in T and B cells.
    Article Snippet: CARD11 acts as a gatekeeper for adaptive immune responses after T cell or B cell antigen receptor (TCR/BCR) ligation on lymphocytes.. PKC / -catalyzed phosphorylation of CARD11 promotes the assembly of the CARD11-BCL10-MALT1 (CBM) complex and lymphocyte activation.. Here, we demonstrated that PKC / -dependent CARD11 phosphorylation also suppressed CARD11 functions in T or B cells.

    Western Blot:

    Article Title: Phosphorylation of serine-893 in CARD11 suppresses the formation and activity of the CARD11-BCL10-MALT1 complex in T and B cells.
    Article Snippet: CARD11 acts as a gatekeeper for adaptive immune responses after T cell or B cell antigen receptor (TCR/BCR) ligation on lymphocytes.. PKC / -catalyzed phosphorylation of CARD11 promotes the assembly of the CARD11-BCL10-MALT1 (CBM) complex and lymphocyte activation.. Here, we demonstrated that PKC / -dependent CARD11 phosphorylation also suppressed CARD11 functions in T or B cells.

    Control:

    Article Title: Modulating cell-free transcription electrolytically with switchable DNA triplexes
    Article Snippet: Before purification, aPCR reaction solutions were loaded into Pierce Streptavidin plates (Thermo Scientific - #15500) that had been previously washed according to the manufacturer’s protocol and left shaking for 4 hours at 400rpm. aPCR reactions were then purified via Qiagen QIAquick PCR purification columns, using spin columns if performed by hand (Qiagen - #28106) or in a 96-well plate vacuum column format if performed by the EGF (Qiagen - #28181). .. Additionally, ssDNA used during the small-scale chemical control tests were not purified via streptavidin pulldown, but were separated via agarose gel electrophoresis, excised and purified using a gel extraction kit (NEB - #T1020S). .. After aPCR, ssDNA products were quantified using QUBIT dye and fluorescence spectrophotometry using either an Agilent Cary Eclipse fluorescence spectrophotometer if using cuvettes or a BMG Clariostar if using plates (excitation = 485nm, emission = 527nm).

    Purification:

    Article Title: Modulating cell-free transcription electrolytically with switchable DNA triplexes
    Article Snippet: Before purification, aPCR reaction solutions were loaded into Pierce Streptavidin plates (Thermo Scientific - #15500) that had been previously washed according to the manufacturer’s protocol and left shaking for 4 hours at 400rpm. aPCR reactions were then purified via Qiagen QIAquick PCR purification columns, using spin columns if performed by hand (Qiagen - #28106) or in a 96-well plate vacuum column format if performed by the EGF (Qiagen - #28181). .. Additionally, ssDNA used during the small-scale chemical control tests were not purified via streptavidin pulldown, but were separated via agarose gel electrophoresis, excised and purified using a gel extraction kit (NEB - #T1020S). .. After aPCR, ssDNA products were quantified using QUBIT dye and fluorescence spectrophotometry using either an Agilent Cary Eclipse fluorescence spectrophotometer if using cuvettes or a BMG Clariostar if using plates (excitation = 485nm, emission = 527nm).

    Agarose Gel Electrophoresis:

    Article Title: Modulating cell-free transcription electrolytically with switchable DNA triplexes
    Article Snippet: Before purification, aPCR reaction solutions were loaded into Pierce Streptavidin plates (Thermo Scientific - #15500) that had been previously washed according to the manufacturer’s protocol and left shaking for 4 hours at 400rpm. aPCR reactions were then purified via Qiagen QIAquick PCR purification columns, using spin columns if performed by hand (Qiagen - #28106) or in a 96-well plate vacuum column format if performed by the EGF (Qiagen - #28181). .. Additionally, ssDNA used during the small-scale chemical control tests were not purified via streptavidin pulldown, but were separated via agarose gel electrophoresis, excised and purified using a gel extraction kit (NEB - #T1020S). .. After aPCR, ssDNA products were quantified using QUBIT dye and fluorescence spectrophotometry using either an Agilent Cary Eclipse fluorescence spectrophotometer if using cuvettes or a BMG Clariostar if using plates (excitation = 485nm, emission = 527nm).

    Gel Extraction:

    Article Title: Modulating cell-free transcription electrolytically with switchable DNA triplexes
    Article Snippet: Before purification, aPCR reaction solutions were loaded into Pierce Streptavidin plates (Thermo Scientific - #15500) that had been previously washed according to the manufacturer’s protocol and left shaking for 4 hours at 400rpm. aPCR reactions were then purified via Qiagen QIAquick PCR purification columns, using spin columns if performed by hand (Qiagen - #28106) or in a 96-well plate vacuum column format if performed by the EGF (Qiagen - #28181). .. Additionally, ssDNA used during the small-scale chemical control tests were not purified via streptavidin pulldown, but were separated via agarose gel electrophoresis, excised and purified using a gel extraction kit (NEB - #T1020S). .. After aPCR, ssDNA products were quantified using QUBIT dye and fluorescence spectrophotometry using either an Agilent Cary Eclipse fluorescence spectrophotometer if using cuvettes or a BMG Clariostar if using plates (excitation = 485nm, emission = 527nm).



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    Thermo Fisher streptavidin pulldown samples
    Validation of TurboID system in glioblastoma primary cell lysates and extracellular vesicles. (A) Adaptation of reaction for TurboID to covalently label proximal proteins. (B) TurboID plasmid used for lentiviral transduction. (C) Depiction of an extracellular vesicle with TurboID and biotinylated protein cargo. Created with BioRender.com . (D) Immunofluorescence validation of TurboID system in GBM612 cells after the addition of 50 µM biotin in the media for 1 h. V5 was used to see expression of TurboID in the cells and <t>streptavidin</t> AF647 to see biotinylation. Scale bars = 50 µm. (E) Western blot validation of the TurboID system in cell lysates of GBM612 cells after the addition of 50 µM biotin in the media for 48 h. V5 was used to probe for the TurboID tag, Streptavidin HRP for biotinylated proteins, and B‐actin for loading control. (F) Summary plot of EVs size modes in nanometers of the GBM612 wt and TurboID expressing cells. All size modes fall into the category of smaller EVs and are <150 nm, and there is no significant difference in size across the conditions. n = 4–6. (G) Summary plot of particles/mL of the GBM612 wt and TurboID expressing cells, showing no significant difference in concentration of EVs across the conditions. n = 4–6. (H) Western blot validation of the TurboID system in EVs of GBM612 cells after the addition of 50 µm biotin in the media for 48 h. V5 was used to probe for the TurboID tag, Streptavidin HRP for biotinylated proteins, and CD63 for EV loading control.
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    (A) Schematic of the experimental strategy integrating label-free phosphoproteomic profiling with miniTurbo-based BioID proximity labeling to investigate NUAK2 function in NEPC. (B, C) Volcano plots showing differential phosphopeptides in NCI-H660 cells treated with the NUAK2 inhibitors HTH-02-006 or G1T-28 compared with DMSO. (D, E) Enrichment analysis of overlapping differentially phosphorylated peptides (DPP) with significantly altered phosphorylation (|log₂FC| ≥ 5) in G1T-28- and HTH-02-006–treated cells relative to DMSO. (F) Bubble plot showing proteins significantly biotinylated by mTb-NUAK2 compared to control(v5-mTb-HcRed). Each bubble represents a distinct protein, with the size of the bubble corresponding to the extent of biotinylation enrichment (number of peptides). (G) Enrichment analysis of proteins identified by NUAK2-dependent biotin labeling reveals significant enrichment of RNA metabolism, RNA splicing, and chromatin remodeling pathways. Enrichment significance determined by hypergeometric test; FDR C<C0.05 considered significant. (H) Venn diagram depicting overlap of proteins differentially phosphorylated (≤ –5 Log2FC, p = 0.01) and significantly biotinylated (≥ 2-fold, p = 0.01) relative to control, identifying 273 candidate NUAK2-associated substrate proteins. (I) Gene set enrichment analysis of overlapping proteins highlights mRNA metabolic processes, cytoskeletal organization, mRNA transport, and chromosome organization. (J) Protein–protein interaction network of the 273 overlapping genes constructed from STRING , BioGrid , OmniPath , and InWeb_IM databases, restricted to experimentally validated interactions and analyzed using Molecular Complex Detection (MCODE) to identify densely connected sub-networks and visualized in Cytoscape. (K) Immunoblot validation of NUAK2 proximity interactions in NCI-H660 cells expressing mTb-NUAK2 or mTb-HcRed controls. Cells were treated with 0.5 mM biotin for 4 hr, followed by streptavidin affinity pulldown to enrich biotinylated proteins. (L-M) Representative immunoblots of co-immunoprecipitations (Co-IP) validating the association of endogenous NUAK2 with core splicing factors in DU-145 and NCI-H660 cells. Schematic in (A) was generated using BioRender.

    Journal: bioRxiv

    Article Title: NUAK2 is a therapeutically tractable regulator of RNA splicing and tumor progression in neuroendocrine prostate cancer

    doi: 10.1101/2025.11.12.687734

    Figure Lengend Snippet: (A) Schematic of the experimental strategy integrating label-free phosphoproteomic profiling with miniTurbo-based BioID proximity labeling to investigate NUAK2 function in NEPC. (B, C) Volcano plots showing differential phosphopeptides in NCI-H660 cells treated with the NUAK2 inhibitors HTH-02-006 or G1T-28 compared with DMSO. (D, E) Enrichment analysis of overlapping differentially phosphorylated peptides (DPP) with significantly altered phosphorylation (|log₂FC| ≥ 5) in G1T-28- and HTH-02-006–treated cells relative to DMSO. (F) Bubble plot showing proteins significantly biotinylated by mTb-NUAK2 compared to control(v5-mTb-HcRed). Each bubble represents a distinct protein, with the size of the bubble corresponding to the extent of biotinylation enrichment (number of peptides). (G) Enrichment analysis of proteins identified by NUAK2-dependent biotin labeling reveals significant enrichment of RNA metabolism, RNA splicing, and chromatin remodeling pathways. Enrichment significance determined by hypergeometric test; FDR C

    Article Snippet: Streptavidin affinity pulldown was performed using PierceTM MS-Compatible Magnetic IP Kit (Thermo Fisher, Catalog Number-90408), following manufacturer’s instructions and samples were eluted in 50 μl elution buffer.

    Techniques: Labeling, Phospho-proteomics, Control, Construct, Western Blot, Biomarker Discovery, Expressing, Co-Immunoprecipitation Assay, Generated

    Validation of TurboID system in glioblastoma primary cell lysates and extracellular vesicles. (A) Adaptation of reaction for TurboID to covalently label proximal proteins. (B) TurboID plasmid used for lentiviral transduction. (C) Depiction of an extracellular vesicle with TurboID and biotinylated protein cargo. Created with BioRender.com . (D) Immunofluorescence validation of TurboID system in GBM612 cells after the addition of 50 µM biotin in the media for 1 h. V5 was used to see expression of TurboID in the cells and streptavidin AF647 to see biotinylation. Scale bars = 50 µm. (E) Western blot validation of the TurboID system in cell lysates of GBM612 cells after the addition of 50 µM biotin in the media for 48 h. V5 was used to probe for the TurboID tag, Streptavidin HRP for biotinylated proteins, and B‐actin for loading control. (F) Summary plot of EVs size modes in nanometers of the GBM612 wt and TurboID expressing cells. All size modes fall into the category of smaller EVs and are <150 nm, and there is no significant difference in size across the conditions. n = 4–6. (G) Summary plot of particles/mL of the GBM612 wt and TurboID expressing cells, showing no significant difference in concentration of EVs across the conditions. n = 4–6. (H) Western blot validation of the TurboID system in EVs of GBM612 cells after the addition of 50 µm biotin in the media for 48 h. V5 was used to probe for the TurboID tag, Streptavidin HRP for biotinylated proteins, and CD63 for EV loading control.

    Journal: Journal of Extracellular Vesicles

    Article Title: TurboID‐Mediated Profiling of Glioblastoma‐Derived Extracellular Vesicle Cargo Proteins

    doi: 10.1002/jev2.70158

    Figure Lengend Snippet: Validation of TurboID system in glioblastoma primary cell lysates and extracellular vesicles. (A) Adaptation of reaction for TurboID to covalently label proximal proteins. (B) TurboID plasmid used for lentiviral transduction. (C) Depiction of an extracellular vesicle with TurboID and biotinylated protein cargo. Created with BioRender.com . (D) Immunofluorescence validation of TurboID system in GBM612 cells after the addition of 50 µM biotin in the media for 1 h. V5 was used to see expression of TurboID in the cells and streptavidin AF647 to see biotinylation. Scale bars = 50 µm. (E) Western blot validation of the TurboID system in cell lysates of GBM612 cells after the addition of 50 µM biotin in the media for 48 h. V5 was used to probe for the TurboID tag, Streptavidin HRP for biotinylated proteins, and B‐actin for loading control. (F) Summary plot of EVs size modes in nanometers of the GBM612 wt and TurboID expressing cells. All size modes fall into the category of smaller EVs and are <150 nm, and there is no significant difference in size across the conditions. n = 4–6. (G) Summary plot of particles/mL of the GBM612 wt and TurboID expressing cells, showing no significant difference in concentration of EVs across the conditions. n = 4–6. (H) Western blot validation of the TurboID system in EVs of GBM612 cells after the addition of 50 µm biotin in the media for 48 h. V5 was used to probe for the TurboID tag, Streptavidin HRP for biotinylated proteins, and CD63 for EV loading control.

    Article Snippet: The streptavidin bead pulldown method was adapted from Cho et al. For each condition, 25 μL of streptavidin Dynabeads (Invitrogen 11205D) was added to 1 mL of RIPA buffer, placed on an end‐over‐end rotator for 2 min, then on a magnetic rack, and supernatant was removed.

    Techniques: Biomarker Discovery, Plasmid Preparation, Transduction, Immunofluorescence, Expressing, Western Blot, Control, Concentration Assay